Explain5 marksChallenge
A student uses a light microscope to observe a stained section of animal tissue. She calibrates an eyepiece graticule using a stage micrometer. The micrometer has 100 divisions per mm. She measures the length of a cell as 40 eyepiece units. At this magnification, each eyepiece unit corresponds to 2.5 µm. She also takes a photomicrograph of the same cell at the same magnification; the cell image in the photomicrograph measures 85 mm.
Define the terms magnification and resolution. Explain why the resolution of an electron microscope is greater than that of a light microscope. State the relationship linking image size, actual size and magnification, and explain which measurement must be converted before the relationship can be used.