CAIE A Level Biology 9700 · Genetic technology
A molecular biologist wishes to produce human growth hormone (hGH) in E. coli. She isolates mRNA from human pituitary cells. Explain how she can use reverse transcriptase to generate a gene for hGH that can be expressed in bacteria.
5 marks, so the mark scheme is looking for about 5 separate creditworthy points. Making the same point twice in different words earns it once.
Explain means: Give the reasons or the mechanism: why or how something happens. Link each point to the next with because, so or which means. A list of facts with no links usually loses the reasoning marks.
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