CAIE A Level Biology 9700 · Genetic technology
In a genetic engineering experiment, a scientist aims to insert a human insulin gene into a bacterial plasmid. Describe the roles of restriction endonucleases and DNA ligase in this process. In your answer, also explain why a promoter may need to be transferred with the insulin gene and how the scientist could confirm that the insulin gene is expressed using a marker gene coding for a fluorescent product.
5 marks, so the mark scheme is looking for about 5 separate creditworthy points. Making the same point twice in different words earns it once.
Describe means: Say what happens or what something is like, in order and with detail. You are not being asked why. If there is data, quote it: name the trend, then give figures with units at the points where it changes.
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