Outline7 marksChallenge
In an experiment, isolated chloroplasts are exposed to light and supplied with ADP and NADP+. A specific inhibitor is added that prevents the movement of protons from the thylakoid space into the stroma through ATP synthase.
(a) Outline the normal process of proton movement across the thylakoid membrane during the light-dependent stage, and explain how this proton movement is coupled to ATP synthesis. (b) Predict and explain the effect of the inhibitor on the production of triose phosphate (TP) in the Calvin cycle.