A student makes a temporary preparation of onion epidermal cells for viewing with a light microscope. The eyepiece graticule has 100 divisions. The stage micrometer has 100 divisions per mm.
Describe how the student would calibrate the eyepiece graticule and use it to measure the actual length of an onion epidermal cell in micrometres. In your answer, include a definition of resolution and explain why light microscopes cannot resolve organelles such as ribosomes.