Compare5 marksStandard
An enzyme, arginase, was studied in both free solution and immobilised in alginate beads. The activity (rate of product formation, arbitrary units) was measured at various pH values using appropriate buffer solutions. The results are shown in the table below.
| pH | Activity of free arginase (units) | Activity of immobilised arginase (units) |
|---|---|---|
| 4.0 | 5 | 15 |
| 5.0 | 20 | 35 |
| 6.0 | 50 | 60 |
| 7.0 | 80 | 75 |
| 8.0 | 80 | 75 |
| 9.0 | 40 | 50 |
| 10.0 | 10 | 30 |
Compare the effect of pH on the activity of the free and immobilised forms of arginase.