Outline7 marksChallenge
A researcher aims to produce human growth hormone (HGH) in Escherichia coli. She has isolated mRNA for HGH from human pituitary cells. Outline the steps required to:
- obtain a gene for HGH from the mRNA;
- insert this gene into a bacterial plasmid; and
- confirm expression of the gene in transformed bacteria using a fluorescent marker gene.
Include in your answer the roles of named enzymes and any additional DNA sequences that must be transferred with the HGH gene.