A researcher constructs a recombinant plasmid containing a human gene that codes for a therapeutic protein. The plasmid includes a promoter that is recognised by bacterial RNA polymerase. The researcher also inserts a gene encoding green fluorescent protein (GFP) under the control of the same promoter. After transforming bacteria with this plasmid, describe how the researcher could use the GFP marker gene to confirm that the human gene has been taken up and expressed by the bacterial cells.
CAIE A Level Biology 9700 · Genetic technology
Genetic technology: describe question
Describe4 marksStandard
How this question is marked
4 marks, so the mark scheme is looking for about 4 separate creditworthy points. Making the same point twice in different words earns it once.
Describe means: Say what happens or what something is like, in order and with detail. You are not being asked why. If there is data, quote it: name the trend, then give figures with units at the points where it changes.
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