A tomato plant in a garden is observed on a hot, dry afternoon. The leaves appear wilted, but by the evening they have fully recovered. A biologist explains that the stomata closed during the day to reduce water loss and reopened later.
Outline the mechanism by which guard cells regulate stomatal aperture. In your answer, include the role of abscisic acid (ABA) and calcium ions (Ca²⁺) in closing stomata during water stress.