The following is an mRNA sequence from a eukaryotic gene:
5'- AUG GCU UAC CGA UAA -3'
A partial mRNA codon table is shown:
| Codon | Amino acid |
|---|---|
| AUG | Met (start) |
| GCU | Ala |
| GCG | Ala |
| UAC | Tyr |
| CGA | Arg |
| UAA | stop |
Three separate mutations occur in this sequence:
- A substitution of the uracil in the second codon with guanine.
- A deletion of the first guanine of the third codon.
- An insertion of an extra adenine after the first base of the fourth codon.
Describe how each mutation would affect the polypeptide produced from this mRNA.