CAIE A Level Biology 9700 · Cell membranes and transport

Cell membranes and transport: explain question

Explain5 marksStandard

The fluidity of cell surface membranes can be measured using fluorescence recovery after photobleaching (FRAP). In an experiment, the lateral diffusion of a fluorescent lipid probe was measured in normal human red blood cell membranes and in membranes that had been treated to remove most of the cholesterol. The relative mobility of the probe (indicating membrane fluidity) was recorded at two temperatures:

TemperatureNormal membraneCholesterol-depleted membrane
15°C0.250.10
37°C0.550.70

(Values: higher number = greater fluidity)

Using your knowledge of the fluid mosaic model and the arrangement of cholesterol in cell surface membranes, explain the role of cholesterol in affecting membrane fluidity at different temperatures.

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