Describe5 marksChallenge
A scientist wishes to transfer a gene coding for a fluorescent protein from a jellyfish into a bacterium so that the bacterium produces the fluorescent protein. The scientist has isolated the coding sequence of the gene but it lacks a promoter. Describe why it is necessary to transfer a promoter along with the gene, and outline the steps taken to construct a recombinant plasmid that contains both a suitable promoter and the gene, using restriction endonucleases, DNA ligase, and plasmids. Also explain how expression of the transferred gene can be confirmed.